Principal Investigator

Jennifer A. Doudna
Li Ka Shing Chancellor’s Professor of Biomedical Science

Please refer to the guidelines listed on the "Contact" page to assist with a timely review of your message.
Jennifer A. Doudna
Principal Investigator

Dr. Jennifer Doudna is a member of the departments of Molecular and Cell Biology and Chemistry at UC Berkeley, the Howard Hughes Medical Institute, and Lawrence Berkeley National Lab, along with the National Academy of Sciences, and the American Academy of Arts and Sciences.

Biographical Highlights:

Feel free to use this short bio and picture for announcements.

Postdoctoral Associates

Brady Cress
NIH Kirschstein-NRSA (F32) Postdoctoral Fellowship Awardee
Brady Cress

Diverse CRISPR-Cas systems are now known to function as integral components of the immune repertoire of many microorganisms, with the currently known catalog of systems spanning two of the three domains of life and contributing to the capacity of these bacteria and archaea to thwart viral infection. Eukaryotes conspicuously lack endogenous CRISPR-Cas systems, but it is not yet known if these molecular surveillance complexes can be co-opted to achieve therapeutically relevant inhibition of viral infection in humans through direct interference with the genomes of human viruses. While investigating strategies to improve the therapeutic potential of CRISPR-Cas components, I will also examine our ability to temporally control the editing activity of diverse CRISPR effectors.

Jennifer Hamilton
Jane Coffin Childs Memorial Fund for Medical Research Postdoctoral Fellowship Awardee
Jennifer Hamilton

CRISPR-Cas-based genome editing tools enable the control of gene expression in cells, tissues and whole organisms. Although invaluable for experimental studies, translation of these advances into clinical therapeutics requires delivery of CRISPR-Cas proteins and guide RNA to disease-relevant organs in the body. All current in vivo delivery strategies have drawbacks including ineffective delivery to target tissue, prolonged nuclease expression leading to off-target damage, and clearance of edited cells by adaptive immune responses. I posit that viral infection strategies can be harnessed to overcome the challenges faced by the in vivo delivery of genome editing tools. In the Doudna laboratory, I am applying my background in viral engineering to create the next-generation of CRISPR-Cas delivery vehicles and translate these technologies into therapeutics. By merging virology with bioengineering, I aim to make these revolutionary genome-based treatments accessible to all people who can benefit.

Tina Liu
NIH Kirschstein-NRSA (F32) Postdoctoral Fellowship Awardee
Tina Liu

CRISPR-Cas systems are an ancient and widespread RNA-guided adaptive immune system in bacteria and archaea. My research focuses on how multisubunit Type III CRISPR-Cas complexes target transcriptionally active DNA and RNA of invading phages and plasmids. Using a combination of biochemistry and single-particle electron microscopy, I aim to uncover the mechanism of transcription-coupled target recognition by Type III complexes. Understanding how they find and destroy their targets will provide fundamental insights into RNA-guided immunity in prokaryotes, and could potentially lead to a tool that can detect or target actively expressed genes in heterologous systems, such as eukaryotic cells.

Ben Rubin
NIH Kirschstein-NRSA (F32) Postdoctoral Fellowship Awardee
Ben Rubin

CRISPR-Cas in Uncultured Microbes: The large majority of life has never been cultivated within the laboratory. This life can both be mined for new CRISPR-Cas systems and manipulated by these systems to facilitate understanding. My research focuses on the development of genetics, enabled by CRISPR-Cas, in communities of uncultured microorganisms. Secondarily, I look for new CRISPR-CAS and CRISPR-Cas-like defense systems within these same communities.

I-Li Tan

Glioblastoma (GBM) is a deadly disease that most people with this cancer died within two years of diagnosis despite decades of research on finding more effective treatments. With the recent development of CRISPR (clustered regularly interspaced short palindromic repeats) and CRISPR-associated (Cas) proteins as easily accessible and programmable means of editing and regulating genes, I propose to directly leverage CRISPR-Cas as a therapeutic modality to eliminate GBM cells. I have two main research focuses 1) use CRISPR-Cas system to dissect mechanisms of tumorigenesis and identify therapeutic targets, and 2) develop in vivo delivery tools to target GBM stem-like cells, the main population responsible for tumor recurrence, using intracranial xenograft model of GBM.

David Colognori 
Jane Coffin Childs Memorial Fund for Medical Research Postdoctoral Fellowship Awardee
Katarzyna Soczek
Abdullah Syed
NSERC Postdoctoral Fellowship Awardee
Abdullah Syed
Matthew Kan
Pediatric Scientist Development Program Fellow Joint with Jennifer Puck at UCSF
Matthew Kan

Matt is a clinical fellow in allergy and immunology at University of California, San Francisco (UCSF). He has been studying the immune system since he was an undergraduate at Harvard University, where he worked on the role of NKT cells in asthma with Dale Umetsu. He received his MD and PhD in immunology at Duke University in the labs of Carol Colton and Michael Dee Gunn, where he identified a novel immune-mediated mechanism in the pathogenesis of Alzheimer’s disease that is being explored in a clinical trial. Matt studied computational immunology during a postdoctoral fellowship with Atul Butte at UCSF and completed his residency training in pediatrics at UCSF. His clinical focus is on inborn errors in immunity and he is designing CRISPR/Cas-based gene therapy for patients with primary immune deficiencies.

Kai Chen
Petr Skopintsev
Elizabeth (Abby) Stahl
Joint with Savage Lab NIH Kirschstein-NRSA (F32) Postdoctoral Fellowship Awardee
Elizabeth (Abby) Stahl
Postdoctoral Scholar

Abby is a postdoctoral scholar in the California Institute for Quantitative Biosciences. She began studying the host immune response to bioengineered materials during her time as a graduate student at the University of Pittsburgh McGowan Institute for Regenerative Medicine. She now studies how the immune system responds to Cas proteins and delivery vectors to improve the efficacy of gene editing and to further translate the use of CRISPR systems for in vivo applications.

Ben Adler
Ben Adler
Chenglong Xia
Gary Wang
Gary Wang
Honglue Shi
Honglue Shi

Graduate Students

Basem Al-Shayeb
NSF Fellow
Joint with Banfield Lab
Josh Cofsky
NSF Fellow
Joint with Kuriyan Lab
Josh Cofsky

While protein structures are commonly represented as a single set of 3D coordinates, most biological macromolecules rely heavily on conformational flexibility to effect their functions in solution. Cas effector complexes in particular undergo dramatic conformational movements during the process of RNA-guided nucleic acid targeting. I am broadly probing the energetic landscape of these dynamic interference complexes to better understand how their nuclease activity is regulated.

Arturo Ortega
Joint with the Staskawicz Lab
Connor Tsuchida
NIH Kirschstein-NRSA (F31) Predoctoral Fellowship Awardee
Joy Wang
NSF Fellow
Hannah Karp
Hannah Karp
Brittney Thornton
NSF Fellow and joint with the Savage Lab
Brittney Thornton
Vienna Thomas
Joint w/ Nomura Lab
Vienna Thomas
Muntathar Al-Shimary
Muntathar Al-Shimary
Julia Tartaglia

Staff

Kaihong Zhou
Lab Manager
510-643-0108
Enbo Ma
Senior Staff Scientist
510-643-0108
Min Hyung Kang
Project Scientist
Min Hyung Kang
Project Scientist

As a senior research scientist, I have extensive biological research experiences in molecular and cellular biology in plants, E. coli, yeasts, viruses, mammalian, and human diseases such as autoimmune, glaucoma and hyperbilirubinemia. This has increased my ability to perform many different types of research approaches. With this vast experience, I have joined Dr. Doudna’s lab as a project scientist in order to accomplish my career goal that is to contribute my experience to improve gene therapy through gene editing such as CRISPR-CAS9.

In this lab, I will focus on the in vivo CRISPR-CAS system by which we will figure out how to deliver our gene therapy complex to tissues/organs, specifically and precisely.

In addition, I have realized that people working together is most important. So I have a motto  ‘work together, make together’ is the best way to move forward and make good progress. Furthermore, I always keep in mind for work progress ‘Let it happen? Make it happen!

Lucie Bardet
Project Manager
Lucie Bardet
Project Manager
Lucie Bardet holds a Master of Science in Bioengineering and a Master of Business Administration from the University of California, Berkeley. Prior to joining the Doudna Lab, Lucie worked in global operations and supervised large-scale projects and product development for biotech and healthcare companies, across four continents. She has also supported multiple startups on their operations since she moved to the Bay area. Lucie speaks four languages, is an avid backpacker, and is passionate about taking up new challenges and bringing people together, at 100 feet underwater or on top of a 23,000-foot mountain.

 

Karen Zhu
Jinjuan Ye
Technician
510-643-0108
Cindy Sandoval Espinoza
Technician
Dylan Smock
Technician
Stephanie Stephens
Technician
Stephanie Stephens
Technician
Rachel Rovinsky
Technician
Rachel Rovinsky
Technician
Kate Miller
Technician
Kate Miller
Technician
Alison Ciling
Technician
Alison Ciling
Technician
Emily K. Meier
Technician
Emily K. Meier
Technician
Evelyn Chen
Bryant Xu

Undergraduate Students

Mohak Jain
Defne Yigci
Defne Yigci
Allison Bien
Sara Smith
Sara Smith
Mihir Shah
Mihir Shah
Kelly Liu
Kelly Liu
Ryan Allen
Ryan Allen
Stephen Jiang
Stephen Jiang

Visiting Scholars

Alumni

Former Postdoctoral Associates

Name
Time In Lab
Position and Location
Tina Liu
2015-2021

Senior Scientist, Beam Therapeutics

Enrique Lin Shiao
2019-2021

Asset Acquisitions Associate, BridgeBio

Patrick Pausch
2019-2021

Investigator, Vilnius University

Gavin Knott
2016-2021

Investigator, Monash University

Haridha Shivram
2019-2021

Bioinformatician, Genentech

Shrutee Jakhanwal
2018-2021

Senior Research Investigator, Syngene

Bastian Minkenberg
2017-2021

Research Scientist, Inari Agriculture

Alexandra Amen
2017-2021

Asset Acquisitions Associate, BridgeBio

Jun-Jie (Gogo) Liu
2016-2020

Assistant Professor, Tsinghua University

Elizabeth O'Brien
2018-2020

Bioscience Sales Specialist for Nikon, Inc.

Audrone Lapinaite
2015-2019

Assistant Professor, Arizona State University

Fuguo Jiang
2013-2018

In Memoriam of Fuguo Jiang; Assistant Professor, UT MD Anderson Cancer Center, Jiang Lab

Kyle Watters
2016-2018

Senior Bioengineer, Arbor Biotechnologies

Christof Fellmann
2015-2018

Assistant Adjunct Professor, Department of Cellular & Molecular Pharmacology, School of Medicine, University of California, San Francisco; Staff Research Investigator, Gladstone Institutes

Brett Staahl
2013-2018

Co-founder, Scribe Therapeutics

Chun-Hao Huang
2016-2018
Natalia Orlova
2017-2018

Process Development Scientist, Grifols Therapeutics

David Burstein
2015-2018

Assistant Professor, Tel Aviv University

Stephen Floor
2011-2017

Assistant Professor, UCSF

Mitchell O'Connell
2013-2017
Romain Rouet
2015-2017
April Pawluk
2016-2017
David Taylor
2014-2016

Assistant Professor, University of Texas, Austin

Emine Kaya
2012-2016
Philip Kranzusch
2012-2016

Assistant Professor, Harvard Medical School, Kranzusch Lab

Nathanael Lintner
2011-2016

Senior Scientist, Pfizer

Yun Bai
2011-2015

Assistant Professor, ShanghaiTech

Steven Lin
2012-2015

Assistant Research Fellow, Academica Sinica

Ross Wilson
2010-2015

Principal Investigator, Wilson Lab, UC Berkeley

Ho Young Lee
2008-2013

Scientist at Genentech

Stefanie Mortimer
2009-2013

Senior Manager, Technology Development at Guardant Health

Aaron Brewster
2010-2013

Project Scientist, Berkeley Lab

Martin Jinek
2007-2012

Assistant Professor, Institute of Biochemistry, University of Zurich

Monika Martick
2011-2012

Scientist, Miroculus

Blake Wiedenheft
2007-2012

Assistant Professor, Montana State University

Dipali Sashital
2008-2012

Assistant Professor, Iowa State University

Andrew Mehle
2005-2011

Assistant Professor, Univ. of Wisconsin, Madison

Sandro Ataide
2006-2008

Lecturer, Molecular & Microbial Biosciences, The University of Sydney

Ryuya Fukunaga
2007-2008

Assistant Professor of Biological Chemistry, Johns Hopkins University School of Medicine

Euiyoung Bae
2007-2008

Assistant Professor, Seoul National University

Karin Felderer
2006-2008

Associate Director /Laboratory Leader-Protein Production, MorphoSys AG

Glen Borchert
2007-2008

Assistant Professor, University of Southern Alabama

Si Wu
2005-2008
Director of Research and Development
Suzhou Elite Force Biosciences Co. Ltd.
Wendy V. Gilbert
2004-2008

Associate Professor, MIT

Chris S. Fraser
2003-2008

Associate Professor, UC Davis

Nik H. Chmiel
2002-2007

Staff Scientist, Bio-Rad Laboratories

Ian J. MacRae
2002-2007
Katrin Karbstein
2003-2006

Associate Professor, The Scripps Research Institute

Rich Spanggord
2002-2006

Senior Scientist, Baxalta, Inc.

Ailong Ke
2002-2005

Associate Professor, Cornell University

Li Chen
2001-2005

Sigma-Aldrich, Shanghai, China

Peter Adams
2000-2002

Research Scientist, NIH

Bidya Sagar
2000-2002

Service Architect,Hitachi Consulting

Jeremy M. Murray
1999-2002

Staff Scientist, Genentech

Benoit Masquida
1999-2000

Research Director, University of Strasbourg, Strasbourg, France

Jeffrey S. Kieft
1998-2001
Robert Batey
1997-2001
Adrian Ferré-D'Amaré
1995-1999

Lab Head, NIH

Sonia DeMorais
1995-1996

Former Graduate Students

Name
Time In Lab
Position and Location
Emeric Charles
2017-2021

Scientist, Scribe Therapeutics

Marbo Lobba
2015 -2020

CEO/Co-Founder at Catena Biosciences

Jorge Bardales Mendieta
2014-2018
Addison Wright
2013-2018
Lucas Harrington
2015-2018

Co-founder, Chief Discovery Officer, Mammoth Biosciences

Janice Chen
2015-2018

Co-founder, Chief Research Officer, Mammoth Biosciences

Steven Strutt
2014-2018

Scientist, Spotlight Therapeutics

Akshay Tambe
2013-2017

Scientist, Spotlight Therapeutics

Benjamin Oakes
2014-2017

Entrepreneurial Fellow, Oakes Lab, Innovative Genomics Institute

Kevin Doxzen
2013-2017

Science Media Communications Innovative Genomics Institute

Alexandra Seletsky
2012-2017
Spencer Knight
2014-2017

Data Scientist Forsite Capital

Megan Hochstrasser
2012-2016

Communications Manager Innovative Genomics Institute

James Nunez
2012-2016

Postdoc, Weissman lab, UCSF

Stephen Wilson
2012-2016

Postdoc, Susan Lindquist lab, MIT

Sam Sternberg
2009-2015

Assistant Professor, Columbia University

Mary Anne Kidwell
2009-2014

Consultant, Boston Consulting Group

Cameron Noland
2007-2012

Senior Scientific Researcher, Genentech

Mark Luskus
2011-2012

Freelance Marketer, Boulder Colorado

Rachel Haurwitz
2008-2012

President/CEO, Caribou Biosciences

Bryan Clarkson
2007-2011
Katherine Berry
2006-2011

Assistant Professor, Mount Holyhoke College

Amy Weeks
2008

Postdoctoral Fellow, UCSF

Fai Y. Siu
2003-2008

Postdoc. Research Assoc., R. Stevens Lab, Scripps Research Institute

Eric M. Friedman
2004-2007
Adrian Repic
2003-2006

Resident, Virginia Commonwealth University Department of Radiology

Bunpote Siridechadilok
2002-2006

Research Scientist, Siriraj Hospital, Thailand

Dennis Lullo
2003-2005

Quality Engineer II, Lifecell

Lisa Valdin
2002-2005

Director of Marketing Azure Biosystems, Inc

Kristi Pullen
2001-2002

Staff Scientist, Health Program National Resources Defense Council

Angie Grech
1999-2001

Global Director of Customer Success, LinkedIn Learning

Miguel Talavera
1998-2002

Scientist-Process Development-Attribute Sciences Amgen , Yale U.

Robert Rambo
1997-2003

Principal Beamline Scientist Diamond Light Source, Yale U.

Andrej Luptak
1997-2002

Associate Professor, UC Irvine

Lan Zhang
1997-2002

Principal Scientist, Merck & Co., Inc.

Daniel Battle
1996-2002

Asst. Professor, Ohio State University

Rebecca Hanna
1996-2000

Owner and Proprietor, MadeWithMolecules.com

Elizabeth Doherty
1995-2000

Technical Specialist, Washington DC

Jamie Cate
1994-1997

Prof., UC Berkeley